|
KMID : 0364819910290020092
|
|
Korean Journal of Microbiology
1991 Volume.29 No. 2 p.92 ~ p.96
|
|
|
Purification Cytochrome c-551 from Rhodopseudomonas Bacterium, Rhodopseudomonas gelatinosa ATCC 17013
|
|
Kang, Dae Gil
|
|
|
Abstract
|
|
|
1
The soluble cytochrome c-551 of photosynthetic bacterium, Rhodopseudomonas gelatinosa ATCC 17013 was purified through a sequence of four step chromatography including CM-cellulose ion-exchange chromatography, DEAE-Sephacel chromatography, Sephacryi s-200 gel permeation chromatography, and HPLC (SP-5PW). The molecular weight of the purified cytochrome c-551 was 14,600 Da, and this protein shows the absorption peak at 551 nm, 522 nm, and 417 nm as the reduced form, and at 412 um as the oxidized form. The cytochrome c551 seems to be a substrate for the terminal oxidase in the electron transport chain.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|